| Tool name | Easyexon Primer |
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| URL | http://129.43.22.27/~primer/EasyExonPrimer.html |
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| Important features | 1. Allows the user to design primers specific to all exons or coding exons only.
2. Displays the exon sequence with SNP marking
3. Displays the binding pattern between the primers and the target. |
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| Citations | Wu X, Munroe DJ. EasyExonPrimer: automated primer design for exon sequences.
Appl Bioinformatics. 2006;5(2):119-20. PubMed PMID: 16722777. |
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| Year of publication | 2006 |
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| Rank by usage frequency | 100 |
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| Comments | The source code is also available upon request. CONTACT: Xiaolin Wu (forestwu@mail.nih.gov). |
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| Function | Junction primers, General PCR |
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| Category | Online, Free |
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| Features | 1. Mask the repeat sequence and SNP sites in the target gene.
2. Displays the gene structure by linking to UCSC genome browser.
3. Allows the user the analyze the primer pairs [that is checking Tm, GC% etc.]. |
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| Description | For amplifying longer DNA fragments. |
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